At 24 hours after the final challenge, the mice were sacrificed, and the BALF was collected, which was separated into the supernatant and cell pellet
At 24 hours after the final challenge, the mice were sacrificed, and the BALF was collected, which was separated into the supernatant and cell pellet. After 2 days, mice received OAA for 1 hour. Evans blue and DNP-HSA mixture was…