Plates were washed with ELISA wash buffer (PBS-1
Plates were washed with ELISA wash buffer (PBS-1.05% Tween-100mM NaCL-0.5mM NaH2 PO4 -1.5mM Na2HPO4), blocked with 1% bovine serum albumin PBS, incubated with immune sera overnight at 40C, and then washed with ELISA wash buffer. anti-PspA, anti-PspC, anti-PcpA, and anti-PLY…