As shown in Figure 6, ?Delta Ct increased monotonically with the concentration of hCG; we estimated the limit of detection (LOD) at 25 pg/ml (signal higher than the no-hCG control plus 3 times the standard deviation of the no-hCG control; 660 fM; 100 l sample volume)
As shown in Figure 6, ?Delta Ct increased monotonically with the concentration of hCG; we estimated the limit of detection (LOD) at 25 pg/ml (signal higher than the no-hCG control plus 3 times the standard deviation of the no-hCG control;…